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	Provedor de dados BJMBR (2) Autor Cerutti,J.M. (2) Andrade,J.A.D. (1) Dalla Torre,C.A. (1) Maciel,R.M.B. (1) Pinheiro,N.A. (1) Riggins,G.J. (1) Souza,S.J. de (1) Villa,L.L. (1) de Toledo,S.R.C. (1) Mais... Palavra-chave Cancer (1) Gene expression profiling (1) Human thyroid cancer (1) Leukemia (1) SAGE (1) Serial analysis of gene expression (1) Silver staining (1) TRAP assay (1) Telomerase (1) Telomeric repeat amplification protocol (1) Tumor antigens (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2003 (1) 2002 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines BJMBR Dalla Torre,C.A.; Maciel,R.M.B.; Pinheiro,N.A.; Andrade,J.A.D.; de Toledo,S.R.C.; Villa,L.L.; Cerutti,J.M.. Measurement of telomerase activity in clinically obtained tumor samples may provide important information for use as both a diagnostic marker and a prognostic indicator for patient outcome. In order to evaluate telomerase activity in tumor tissue without radiolabeling the product, we developed a simple telomeric repeat amplification protocol-silver-staining assay that is less time-consuming, is safe and requires minimal equipment. In addition, we determined the sensitivity of the silver-staining method by using extracts of telomerase-positive thyroid carcinoma cell lines which were serially diluted from 5,000 to 10 cells. Telomerase activity was also assayed in 19 thyroid tumors, 2 normal controls and 27 bone marrow aspirates. The results indicate that the... Tipo: Info:eu-repo/semantics/other Palavras-chave: Telomeric repeat amplification protocol; TRAP assay; Human thyroid cancer; Leukemia; Telomerase; Silver staining. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100009 What can digital transcript profiling reveal about human cancers? BJMBR Cerutti,J.M.; Riggins,G.J.; Souza,S.J. de. Important biological and clinical features of malignancy are reflected in its transcript pattern. Recent advances in gene expression technology and informatics have provided a powerful new means to obtain and interpret these expression patterns. A comprehensive approach to expression profiling is serial analysis of gene expression (SAGE), which provides digital information on transcript levels. SAGE works by counting transcripts and storing these digital values electronically, providing absolute gene expression levels that make historical comparisons possible. SAGE produces a comprehensive profile of gene expression and can be used to search for candidate tumor markers or antigens in a limited number of samples. The Cancer Genome Anatomy Project has... Tipo: Info:eu-repo/semantics/article Palavras-chave: Serial analysis of gene expression; SAGE; Tumor antigens; Gene expression profiling; Cancer. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800003 Registros recuperados: 2 Primeira ... 1 ... Última

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